Ashley W. Johnston, MD
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Being trusted by families to care for their children is an incredible honor that I never take for granted.
– Ashley W. Johnston, MD

Ashley W. Johnston, MD

Pediatric Urologist, Urologist

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Where I See Patients
Duke Ambulatory Surgery Center - Durham, NC
Duke Children's Health Center Urology Clinic - Durham, NC
Duke Children's Specialty Services of Greensboro - Greensboro, NC
Duke Pediatric Urology South Durham - Durham, NC
Lenox Baker Children's Hospital - Durham, NC
Being trusted by families to care for their children is an incredible honor that I never take for granted.
– Ashley W. Johnston, MD

About Me

As a pediatric urologist, I specialize in disorders of the kidneys, ureters, bladder, and genitalia. I medically and surgically treat all conditions in pediatric urology including undescended testicles, foreskin issues, hypospadias, vesicoureteral reflux, hydronephrosis, kidney stones, and bladder dysfunction. I am particularly passionate about helping children with complex genitourinary birth defects, neurogenic bladder/bowel due to spinal disorders such as spina bifida, and disorders of sex differentiation. Additionally, I offer minimally invasive approaches including robotic surgery. Although I meet most of my patients during childhood, I follow many through adolescence and even into adulthood building strong relationships with both them and their families. As a mother, I know how scary doctors visits especially those involving surgery, can be for children and parents. By engaging both families and children in their healthcare experience, we can work as a team creating a plan that always prioritizes the health and happiness of the patient. Being trusted to care for a child is a great privilege as I know that each child is someone’s favorite person.

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School of Medicine Departments and Divisions
Current Appointments and Affiliations
Languages I Speak

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Training and Education

Pediatric Urology, Riley Hospital for Children, Indiana University, 2023
Robotics and Innovative Technology Research, Harvard Medical School/Boston Children's Hospital, 2015
Urology, Duke University, 2015-2021
MD, University of Maryland, 2014

Clinical Focus and Research

My Research Interests

My research focuses on patient-centered outcomes such as quality of life, satisfaction, and decisional regret in pediatric urology particularly for patients who’ve undergone major genitourinary reconstruction. I hope to aid families in their surgical decision making to ease their burden as surrogate decision makers and to ensure children maintain their health and wellbeing as adults. Secondarily, I am passionate about medical education designing curricula incorporates novel medical research and promotes compassionate patient care.


Insurance Accepted

Duke Health contracts with most major health insurance carriers and transplant networks, including the ones listed below.

    • Aetna Choice POS, Aetna Choice POS II
    • Aetna Elect Choice HMO, Aetna Open Access Elect Choice
    • Aetna Health Network Only, Aetna Health Network Option
    • Aetna HMO
    • Aetna Limited Benefit Insurance PPO
    • Aetna Managed Choice POS
    • Aetna Medicare Assure Plan (HMO D-SNP)
    • Aetna Medicare Eagle Plan (PPO)
    • Aetna Medicare Essential Plan (PPO)
    • Aetna Medicare Value Plan (HMO)
    • Aetna Open Access HMO, Open Access Aetna Select, Aetna Open Access Managed Choice
    • Aetna Open Choice PPO
    • Aetna PCP Coordinated POS Plan
    • Aetna Quality Point of Service (QPOS)
    • Aetna Select HMO
    • Aetna Traditional Choice
    • Aetna Voluntary Indemnity Group Plan
    • Aetna Whole Health – Duke WakeMed WKCC
    • Aetna/CVS Health
    • Ambetter of North Carolina
    • Blue Advantage
    • Blue Medicare (HMO, PPO)*
    • Blue Options (123, PPO, HSA)
    • Blue Select
    • NC State Employees Health Plan
    • *Duke HomeCare and Hospice does not participate in the plan.

    • Cigna Behavioral Health (*Limited eligibility)
    • Cigna Choice Plus
    • Cigna Connect Individual Family Plan
    • Cigna Open Access
    • Cigna Open Access Plus
    • *Please call Cigna Behavioral Heath to see if the provider is participating in your plan.

    • Duke Basic
    • Duke Select
    • Experience Health Medicare Advantage (HMO) Plan
  • Gateway Health Alliance
  • Healthgram
    • Humana Choice (PPO)
    • Humana Choice - Medicare Advantage (PPO)
    • Humana ChoiceCare - Medicare Advantage (PPO)
    • Humana Gold Choice - Medicare Advantage (PFFS)
    • Humana Gold Plus - Medicare Advantage (HMO)
    • Humana Medicare Advantage Group Plan - NC State Retirees
  • MedCost
    • First Medicare Direct
    • Medicare Part A
    • Medicare Part B
    • AmeriHealth Caritas North Carolina
    • Carolina Complete Health
    • Healthy Blue
    • NC Medicaid Direct
    • WellCare of North Carolina
    • TRICARE Prime
    • TRICARE Prime Remote
    • TRICARE Select
    • AARP Medicare Advantage Plan 2 (HMO-POS)
    • AARP Medicare Complete (HMO, PPO)*
    • AARP Medicare Complete Essential (HMO)*
    • All Savers Alternate Funding
    • All Savers Fully Insured
    • United Healthcare (HMO, PPO, POS)
    • United Healthcare Charter/Charter Balance/Charter Plus
    • United Healthcare Choice/Choice Plus
    • United Healthcare Core/Core Essential
    • United Healthcare Navigate/Navigate Plus/Navigate Balanced
    • United Healthcare Option PPO
    • United Healthcare Passport Connect Choice/Choice Plus
    • United Healthcare Passport Connect Options PPO
    • United Healthcare Select/Select Plus
    • United Healthcare Shared Services - Harvard Pilgrim/UHC Options PPO Network
    • United Medical Resources (UMR)
    • *Duke HomeCare and Hospice and mental health providers do not participate with the plan.

Before scheduling your appointment, we strongly recommend you contact your insurance company to verify that the Duke Health location or provider you plan to visit is included in your network. Your insurance company will also be able to inform you of any co-payments, co–insurances, or deductibles that will be your responsibility. If you proceed in scheduling an appointment and your health insurance benefits do not participate with Duke, your out of pocket liability may be higher. We will contact you regarding your coverage and patient liability. If you are uninsured, learn more about our financial assistance policy.

External Relationships

Duke requires faculty members to report certain relationships outside of their employment in order to ensure potential conflicts can be addressed. This faculty member has reported no outside activities that relate to their Duke responsibilities.