Physicians

Department / Division
Pathology / Pathology Clinical Services

Address
DUMC 3712
Durham, NC 27710

Office Telephone
919-684-6965

Fax Telephone
919-684-6962

Training

• MD, Duke University School of Medicine, 1999

Residency

• Pathology, Duke University Medical Center, 2004

Other Training

• PhD, Molecular Cancer Biology, Duke University Medical Center, 1999

Clinical Interests
Molecular diagnostic testing

Research Interests
Dr. Datto is a AP/CP/MGP board certified pathologist who specializes in molecular pathology.  He is director of the Duke University Health System Clinical Molecular Diagnostics Laboratory.  This CAP/CLIA certified laboratory does the majority of the DNA and RNA based high complexity molecular diagnostic testing for Duke patients and health care providers.  Testing includes full gene sequencing and targeted mutation analysis for inherited diseases, targeted mutation and polymorphism analysis for somatic mutations in a variety of tumor types and disease conditions for prognostics and pharmacogenomic applications and quantitative analysis for viral load and minimal residual disease testing in patients with leukemias and lymphomas.  

Dr. Datto also has an independent research program developing multigene assays to determine cancer patient specific prognoses in the context of breast and colon cancer with the ultimate goal of producing improved molecular diagnostic assays. The ultimate goal of this truly translational effort is the incorporation of genome wide expression profiling for prognosis and diagnosis into the clinical laboratories for routine patient care. This involves the study of factors that affect the precission and accuracy array-based predictors, as well as deriving novel diagnostic molecular targets.  Finally, Dr. Datto has a strong research interest in resident and medical student education, specifically the development of web-base community tools for anatomic and clinical pathology education.

Industry Relationships and Collaborations (What's this?)

This physician (or a member of their immediate family) has a working relationship (i.e. consulting, research, and/or educational services) with the companies listed below. These relations have been reported to the health system leadership and, when appropriate, management plans are in place to address potential conflicts.

• Affymetrix, Inc.
• Almac Diagnostics LLC
• McKesson Corporation
• Oakstone Publishing CME

Representative Publications
Barry WT, Kernagis DN, Dressman HK, Griffis RJ, Hunter JD, Olson JA, Marks JR, Ginsburg GS, Marcom PK, Nevins JR, Geradts J, Datto MB. Intratumor heterogeneity and precision of microarray-based predictors of breast cancer biology and clinical outcome. J Clin Oncol. 2010 May 1;28(13):2198-206. (2010) Abstract

Crow J, Youens K, Michalowski S, Perrine G, Emhart C, Johnson F, Gerling A, Kurtzberg J, Goodman BK, Sebastian S, Rehder CW, Datto MB. Donor Cell Leukemia in Umbilical Cord Blood Transplant Patients. A Case Study and Literature Review Highlighting the Importance of Molecular Engraftment Analysis. J Mol Diagn. 2010 Apr 29. (2010) Abstract

Gatza ML, Lucas JE, Barry WT, Kim JW, Wang Q, Crawford MD, Datto MB, Kelley M, Mathey-Prevot B, Potti A, Nevins JR. A pathway-based classification of human breast cancer. Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6994-9. (2010) Abstract

Pan H, Ding E, Hu M, Lagoo AS, Datto MB, Lagoo-Deenadayalan SA. SMAD4 is required for development of maximal endotoxin tolerance. J Immunol. 2010 May 15;184(10):5502-9. (2010) Abstract

Schilling SH, Hjelmeland AB, Radiloff DR, Liu IM, Wakeman TP, Fielhauer JR, Foster EH, Lathia JD, Rich JN, Wang XF, Datto MB. NDRG4 is required for cell cycle progression and survival in glioblastoma cells. J Biol Chem. 2009 Sep 11;284(37):25160-9. (2009) Abstract

Horbinski C, Dacic S, McLendon RE, Cieply K, Datto M, Brat DJ, Chu CT. Chordoid Glioma: A Case Report and Molecular Characterization of Five Cases. Brain Pathol. 2008 Jul 23. (2008) Abstract

Ma C, Rong Y, Radiloff DR, Datto MB, Centeno B, Bao S, Cheng AW, Lin F, Jiang S, Yeatman TJ, Wang XF. Extracellular matrix protein betaig-h3/TGFBI promotes metastasis of colon cancer by enhancing cell extravasation. Genes Dev. 2008 Feb 1;22(3):308-21. (2008) Abstract

Wooldridge AA, Fortner CN, Lontay B, Akimoto T, Neppl RL, Facemire C, Datto MB, Kwon A, McCook E, Li P, Wang S, Thresher RJ, Miller SE, Perriard JC, Gavin TP, Hickner RC, Coffman TM, Somlyo AV, Yan Z, Haystead TA. Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle. J Biol Chem. 2008 Apr 25;283(17):11850-9. (2008) Abstract

Kwiek NC, Thacker DF, Datto MB, Megosh HB, Haystead TA. PITK, a PP1 targeting subunit that modulates the phosphorylation of the transcriptional regulator hnRNP K. Cell Signal. 2006 Oct;18(10):1769-78. (2006) Abstract

Schilling SH, Datto MB, Wang XF. A phosphatase controls the fate of receptor-regulated Smads. Cell. 2006 Jun 2;125(5):838-40. (2006) Abstract

Datto M, Wang XF. Ubiquitin-mediated degradation a mechanism for fine-tuning TGF-beta signaling. Cell. 2005 Apr 8;121(1):2-4. (2005) Abstract

Robboy SJ, Datto MB. Synchronous endometrial and ovarian tumors: metastatic disease or independent primaries? Hum Pathol. 2005 Jun;36(6):597-9. (2005) Abstract

Moore-Maxwell CA, Datto MB, Hulette CM. Chemotherapy-induced toxic leukoencephalopathy causes a wide range of symptoms: a series of four autopsies. Mod Pathol. 2004 Feb;17(2):241-7. (2004) Abstract

Zhao J, Shi W, Wang YL, Chen H, Bringas P Jr, Datto MB, Frederick JP, Wang XF, Warburton D. Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice. Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L585-93. (2002) Abstract

Borton AJ, Frederick JP, Datto MB, Wang XF, Weinstein RS. The loss of Smad3 results in a lower rate of bone formation and osteopenia through dysregulation of osteoblast differentiation and apoptosis. J Bone Miner Res. 2001 Oct;16(10):1754-64. (2001) Abstract

Decesse JT, Medjkane S, Datto MB, Crémisi CE. RB regulates transcription of the p21/WAF1/CIP1 gene. Oncogene. 2001 Feb 22;20(8):962-71. (2001) Abstract

Datto M, Wang XF. The Smads: transcriptional regulation and mouse models. Cytokine Growth Factor Rev. 2000 Mar-Jun;11(1-2):37-48. (2000) Abstract

Billon N, Carlisi D, Datto MB, van Grunsven LA, Watt A, Wang XF, Rudkin BB. Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation. Oncogene. 1999 May 6;18(18):2872-82. (1999) Abstract

Datto MB, Frederick JP, Pan L, Borton AJ, Zhuang Y, Wang XF. Targeted disruption of Smad3 reveals an essential role in transforming growth factor beta-mediated signal transduction. Mol Cell Biol. 1999 Apr;19(4):2495-504. (1999) Abstract

Haapajärvi T, Kivinen L, Heiskanen A, des Bordes C, Datto MB, Wang XF, Laiho M. UV radiation is a transcriptional inducer of p21(Cip1/Waf1) cyclin-kinase inhibitor in a p53-independent manner. Exp Cell Res. 1999 Apr 10;248(1):272-9. (1999) Abstract

Kivinen L, Tsubari M, Haapajärvi T, Datto MB, Wang XF, Laiho M. Ras induces p21Cip1/Waf1 cyclin kinase inhibitor transcriptionally through Sp1-binding sites. Oncogene. 1999 Nov 4;18(46):6252-61. (1999) Abstract

Liberati NT, Datto MB, Frederick JP, Shen X, Wong C, Rougier-Chapman EM, Wang XF. Smads bind directly to the Jun family of AP-1 transcription factors. Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4844-9. (1999) Abstract

Rich JN, Zhang M, Datto MB, Bigner DD, Wang XF. Transforming growth factor-beta-mediated p15(INK4B) induction and growth inhibition in astrocytes is SMAD3-dependent and a pathway prominently altered in human glioma cell lines. J Biol Chem. 1999 Dec 3;274(49):35053-8. (1999) Abstract

Wong C, Rougier-Chapman EM, Frederick JP, Datto MB, Liberati NT, Li JM, Wang XF. Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the c-Jun promoter by transforming growth factor beta. Mol Cell Biol. 1999 Mar;19(3):1821-30. (1999) Abstract

Hu PP, Datto MB, Wang XF. Molecular mechanisms of transforming growth factor-beta signaling. Endocr Rev. 1998 Jun;19(3):349-63. (1998) Abstract

Li JM, Datto MB, Shen X, Hu PP, Yu Y, Wang XF. Sp1, but not Sp3, functions to mediate promoter activation by TGF-beta through canonical Sp1 binding sites. Nucleic Acids Res. 1998 May 15;26(10):2449-56. (1998) Abstract

Shen X, Hu PP, Liberati NT, Datto MB, Frederick JP, Wang XF. TGF-beta-induced phosphorylation of Smad3 regulates its interaction with coactivator p300/CREB-binding protein. Mol Biol Cell. 1998 Dec;9(12):3309-19. (1998) Abstract

Datto MB, Hu PP, Kowalik TF, Yingling J, Wang XF. The viral oncoprotein E1A blocks transforming growth factor beta-mediated induction of p21/WAF1/Cip1 and p15/INK4B. Mol Cell Biol. 1997 Apr;17(4):2030-7. (1997) Abstract

Yingling JM, Datto MB, Wong C, Frederick JP, Liberati NT, Wang XF. Tumor suppressor Smad4 is a transforming growth factor beta-inducible DNA binding protein. Mol Cell Biol. 1997 Dec;17(12):7019-28. (1997) Abstract

Datto MB, Li Y, Panus JF, Howe DJ, Xiong Y, Wang XF. Transforming growth factor beta induces the cyclin-dependent kinase inhibitor p21 through a p53-independent mechanism. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5545-9. (1995) Abstract

Datto MB, Yu Y, Wang XF. Functional analysis of the transforming growth factor beta responsive elements in the WAF1/Cip1/p21 promoter. J Biol Chem. 1995 Dec 1;270(48):28623-8. (1995) Abstract

Schwarz JK, Bassing CH, Kovesdi I, Datto MB, Blazing M, George S, Wang XF, Nevins JR. Expression of the E2F1 transcription factor overcomes type beta transforming growth factor-mediated growth suppression. Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):483-7. (1995) Abstract