Physicians

Department / Division
Radiology / Neuroradiology

Address
DUMC 3808
Durham, NC 27710

Office Telephone
919-684-7409

Fax Telephone
919-684-7168

Training

• MD, Albany Medical College (New York), 1983

Residency

• Neurology, UNC Hospitals, 1984-1987
• Radiology, Massachusetts General Hospital, 1987-1991

Fellowship

• Neuroradiology, Massachusetts General Hospital, 1990-1992

Other Training

• Fellow, American College of Radiology

Clinical Interests
MR diffusion and perfusion imaging, tumor imaging, molecular imaging, novel contrast agents

Research Interests
I have three major research areas:
I. Applications of nanotechnology to treatment of cancer (both CNS and non-CNS) and brain disorders. I have a dual appointment at both Duke University Medical Center (Department of Radiology) and Emory University School of Medicine/Georgia Tech (Departments of Radiology, Oncology and Biomedical Engineering) which allows me to perform nanotechnology-based research at both institutions.
    My research involves design and implementation of nanoparticles and fluorescent molecules for cancer diagnosis and therapy. Although I am trained as a neurologist and neuroradiologist, most of my nanotechnology-based research is oriented towards non-CNS tumors such as breast cancer and sarcomas. In the past few years, my Emory and Georgia Tech colleagues and I have conducted research using animals with naturally-occurring tumors at the University of Georgia College of Veterinary Medicine. This work has focused on the use of a handheld device to detect fluorophores that are administered intravenously prior to surgery. We are presently validating the use of this combination of imaging device and contrast agent to guide surgical resection of tumors.
     I am also interested in development of nanotechnology-based non-invasive and minimally invasive devices that can continuously monitor tumor physiological characteristics and response to therapy. This work is done in conjunction with a number of colleagues in Biomedical Engineering at both Duke and Emory and supported by a number of NIH grants. Finally, I have a strong interest in use of nanotechnology for tissue engineering and regenerative medicine.

II. Brain tumor imaging in cinical trials and experimental studies with specific focus on:
(A) development of forms of molecular imaging of brain tumors that will allow more specific and sensitive means of understanding tumor physiology,
(B) novel methods of perfusion imaging of brain tumors to understand tumor behavior and response to various therapies, and
(C) development of novel contrast agents.

III. Diffusion tensor imaging (an MR technique that measures rate and direction of microscopic water motion) to examine white matter pathways in the brain and spinal cord. This technique, which has many research applications that we are bringing into the clinical realm, is very well-suited for understanding white matter disorders such as childhood leukodystrophies and multiple sclerosis. My recent work has focused on understanding patterns of normal brain development in childhood and adolescence using diffusion tensor imaging (DTI) fo assess white matter tracts at various stages of brain development. In addition, working with colleagues in the Duke Department of Psychiatry, I have performed research for many years in use of DTI to understand changes in neural circuits associated with late-life depression.
     Since 1998, I have mentored third-year students at Duke University School of Medicine (typically one medical student per year) in both DTI research and perfusion imaging research. Although the research techniques are highly advanced, our implementation of various "user-friendly" software programs allows students with little or no prior experience to analyze data in a productive manner. Our research is also well-suited to individuals with advanced computer skills or an interest in biomedical or electrical engineering. Students work closely with research personnel on a daily basis.  They also meet with collaborators from various basic science and clinical departments and me in a laboratory meeting once a week. The focus of these meetings is to plan experiments, refine research methods, discuss experimental results and prepare manuscripts. Students serve as first authors or co-authors on manuscripts based on their specific research project. The results of a number of such projects have been published. Present projects include use of DTI to evaluate the developing human brain, implementation of DTI to understand the microstructure of the normal canine brain (in collaboration with G. Allan Johnson of the Duke Center for In Vivo Microscopy and colleagues at the University of Georgia College of Veterinary Medicine) and correlation of perfusion measurements in human brain tumors obtained using CT and MR perfusion imaging sequences.

Industry Relationships and Collaborations (What's this?)

This physician (or a member of their immediate family) has a working relationship (i.e. consulting, research, and/or educational services) with the companies listed below. These relations have been reported to the health system leadership and, when appropriate, management plans are in place to address potential conflicts.

• Bayer Schering Pharma AG
• Bayer Schering Pharma AG
• Biomedical Systems
• GE Medical Systems
• Millennium Pharmaceuticals, Inc. (Takeda)
• Theradex

Representative Publications
Mohs AM, Mancini MC, Singhal S, Provenzale JM, Leyland-Jones B, Wang MD, Nie S. Hand-held Spectroscopic Device for In Vivo and Intraoperative Tumor Detection: Contrast Enhancement, Detection Sensitivity, and Tissue Penetration. Anal Chem. 2010 Oct 6. (2010) Abstract

Mohs AM, Provenzale JM. Applications of nanotechnology to imaging and therapy of brain tumors. Neuroimaging Clin N Am. 2010 Aug;20(3):283-92. (2010) Abstract

Provenzale JM, Isaacson J, Chen S, Stinnett S, Liu C. Correlation of apparent diffusion coefficient and fractional anisotropy values in the developing infant brain. AJR Am J Roentgenol. 2010 Dec;195(6):W456-62. (2010) Abstract

Crow MJ, Grant G, Provenzale JM, Wax A. Molecular imaging and quantitative measurement of epidermal growth factor receptor expression in live cancer cells using immunolabeled gold nanoparticles. AJR Am J Roentgenol. 2009 Apr;192(4):1021-8. (2009) Abstract

Madden DJ, Spaniol J, Costello MC, Bucur B, White LE, Cabeza R, Davis SW, Dennis NA, Provenzale JM, Huettel SA. Cerebral white matter integrity mediates adult age differences in cognitive performance. J Cogn Neurosci. 2009 Feb;21(2):289-302. (2009) Abstract

Provenzale JM, Ison C, Delong D. Bidimensional measurements in brain tumors: assessment of interobserver variability. AJR Am J Roentgenol. 2009 Dec;193(6):W515-22. (2009) Abstract

Provenzale JM, Silva GA. Uses of nanoparticles for central nervous system imaging and therapy. AJNR Am J Neuroradiol. 2009 Aug;30(7):1293-301. (2009) Abstract

Madden DJ, Spaniol J, Costello MC, Bucur B, White LE, Cabeza R, Davis SW, Dennis NA, Provenzale JM, Huettel SA. Cerebral White Matter Integrity Mediates Adult Age Differences in Cognitive Performance. J Cogn Neurosci. 2008 Jun 19. (2008) Abstract

Sampson JH, Akabani G, Archer GE, Berger MS, Coleman RE, Friedman AH, Friedman HS, Greer K, Herndon JE 2nd, Kunwar S, McLendon RE, Paolino A, Petry NA, Provenzale JM, Reardon DA, Wong TZ, Zalutsky MR, Pastan I, Bigner DD. Intracerebral infusion of an EGFR-targeted toxin in recurrent malignant brain tumors. Neuro Oncol. 2008 Jun;10(3):320-9. (2008) Abstract

Viola RJ, Provenzale JM, Li F, Li CY, Yuan H, Tashjian J, Dewhirst MW. In vivo bioluminescence imaging monitoring of hypoxia-inducible factor 1alpha, a promoter that protects cells, in response to chemotherapy. AJR Am J Roentgenol. 2008 Dec;191(6):1779-84. (2008) Abstract

Beam D, Poe MD, Provenzale JM, Szabolcs P, Martin PL, Prasad V, Parikh S, Driscoll T, Mukundan S, Kurtzberg J, Escolar ML. Outcomes of unrelated umbilical cord blood transplantation for X-linked adrenoleukodystrophy. Biol Blood Marrow Transplant. 2007 Jun;13(6):665-74. (2007) Abstract

Madden DJ, Spaniol J, Whiting WL, Bucur B, Provenzale JM, Cabeza R, White LE, Huettel SA. Adult age differences in the functional neuroanatomy of visual attention: A combined fMRI and DTI study. Neurobiol Aging. 2007 Mar;28(3):459-76. (2007) Abstract

Provenzale JM, Liang L, DeLong D, White LE. Diffusion tensor imaging assessment of brain white matter maturation during the first postnatal year. AJR Am J Roentgenol. 2007 Aug;189(2):476-86. (2007) Abstract

Provenzale JM. Imaging of angiogenesis: clinical techniques and novel imaging methods. AJR Am J Roentgenol. 2007 Jan;188(1):11-23. (2007) Abstract

Provenzale JM, Mukundan S, Barboriak DP. Diffusion-weighted and perfusion MR imaging for brain tumor characterization and assessment of treatment response. Radiology. 2006 Jun;239(3):632-49. (2006) Abstract

Kealey SM, Kim Y, Whiting WL, Madden DJ, Provenzale JM. Determination of multiple sclerosis plaque size with diffusion-tensor MR Imaging: comparison study with healthy volunteers. Radiology. 2005 Aug;236(2):615-20. (2005) Abstract

McGraw P, Liang L, Escolar M, Mukundan S, Kurtzberg J, Provenzale JM. Krabbe disease treated with hematopoietic stem cell transplantation: serial assessment of anisotropy measurements--initial experience. Radiology. 2005 Jul;236(1):221-30. (2005) Abstract

Provenzale JM, Mukundan S, Dewhirst M. The role of blood-brain barrier permeability in brain tumor imaging and therapeutics. AJR Am J Roentgenol. 2005 Sep;185(3):763-7. (2005) Abstract

Provenzale JM, Mukundan S. Getting small is suddenly very big: review of the proceedings of the third annual meeting of the Society for Molecular Imaging. AJR Am J Roentgenol. 2005 Jun;184(6):1736-9. (2005) Abstract

Provenzale JM. Introduction to the AJR Technology Forum: issues, controversies & utility of PET/CT imaging. AJR Am J Roentgenol. 2005 May;184(5 Suppl):Sii. (2005) Abstract

Taylor WD, MacFall JR, Payne ME, McQuoid DR, Steffens DC, Provenzale JM, Krishnan RR. Greater MRI lesion volumes in elderly depressed subjects than in control subjects. Psychiatry Res. 2005 May 30;139(1):1-7. (2005) Abstract

York G, Barboriak D, Petrella J, DeLong D, Provenzale JM. Association of internal carotid artery injury with carotid canal fractures in patients with head trauma. AJR Am J Roentgenol. 2005 May;184(5):1672-8. (2005) Abstract

Holmes TM, Petrella JR, Provenzale JM. Distinction between cerebral abscesses and high-grade neoplasms by dynamic susceptibility contrast perfusion MRI. AJR Am J Roentgenol. 2004 Nov;183(5):1247-52. (2004) Abstract

Kealey SM, Kim Y, Provenzale JM. Redefinition of multiple sclerosis plaque size using diffusion tensor MRI. AJR Am J Roentgenol. 2004 Aug;183(2):497-503. (2004) Abstract

Provenzale JM, McGraw P, Mhatre P, Guo AC, Delong D. Peritumoral brain regions in gliomas and meningiomas: investigation with isotropic diffusion-weighted MR imaging and diffusion-tensor MR imaging. Radiology. 2004 Aug;232(2):451-60. (2004) Abstract

Taylor WD, MacFall JR, Payne ME, McQuoid DR, Provenzale JM, Steffens DC, Krishnan KR. Late-life depression and microstructural abnormalities in dorsolateral prefrontal cortex white matter. Am J Psychiatry. 2004 Jul;161(7):1293-6. (2004) Abstract