Durham, NC 27710
General cardiology, heart failure
Rockman Lab: Molecular Mechanisms of Hypertrophy and Heart Failure
Overall Research Direction: The major focus of this laboratory is to understand the molecular mechanisms of hypertrophy and heart failure. My laboratory uses a strategy that combines state of the art molecular techniques to generate transgenic and gene targeted mouse models, combined with sophisticated physiologic measures of in vivo cardiac function. In this manner, candidate molecules are either selectively overexpressed in the mouse heart or ablated by homologous recombination, which is followed by an in-depth analysis of the physiological phenotype. To model human cardiac disease, we have created several models of cardiac overload in the mouse using both microsurgical techniques and genetic models of cardiac dysfunction.
Areas of Research
1) Signaling: G protein-coupled receptor signaling in hypertrophy and heart failure focusing on the concept of biased signaling of 7 transmembrane receptors.
2) Molecular physiology: In depth physiological analysis of cardiac function in genetically altered mice to understand the role of G protein-coupled receptor signaling pathways on the development of heart failure in vivo.
3) Deletion screens in Drosophila: To detect novel genes important for cardiac function in the adult fly .
This faculty member (or a member of their immediate family) has a working relationship (i.e. consulting, research, and/or educational services) with the companies listed below. These relations have been reported to the health system leadership and, when appropriate, management plans are in place to address potential conflicts.
Kim, IM; Wolf, MJ; Rockman, HA. Gene deletion screen for cardiomyopathy in adult Drosophila identifies a new notch ligand. Circulation Research. 2010;106:1233-1243. (2010) Abstract
Mangmool, S; Shukla, AK; Rockman, HA. beta-Arrestin-dependent activation of Ca(2+)/calmodulin kinase II after beta(1)-adrenergic receptor stimulation. The Journal of Cell Biology. 2010;189:573-587. (2010) Abstract
Fernandez, L; Marchuk, DA; Moran, JL; Beier, DR; Rockman, HA. An N-ethyl-N-nitrosourea mutagenesis recessive screen identifies two candidate regions for murine cardiomyopathy that map to chromosomes 1 and 15. Mammalian Genome. 2009;20:296-304. (2009) Abstract
Lima, B; Lam, GK; Xie, L; Diesen, DL; Villamizar, N; Nienaber, J; Messina, E; Bowles, D; Kontos, CD; Hare, JM; Stamler, JS; Rockman, HA. Endogenous S-nitrosothiols protect against myocardial injury. Proceedings of the National Academy of Sciences of USA. 2009;106:6297-6302. (2009) Abstract
Tilley, DG; Kim, IM; Patel, PA; Violin, JD; Rockman, HA. beta-Arrestin mediates beta1-adrenergic receptor-epidermal growth factor receptor interaction and downstream signaling. Journal of Biological Chemistry. 2009;284:20375-20386. (2009) Abstract
Kim, IM; Tilley, DG; Chen, J; Salazar, NC; Whalen, EJ; Violin, JD; Rockman, HA. Beta-blockers alprenolol and carvedilol stimulate beta-arrestin-mediated EGFR transactivation. Proceedings of the National Academy of Sciences of USA. 2008;105:14555-14560. (2008) Abstract
Noma, T; Lemaire, A; Naga Prasad, SV; Barki-Harrington, L; Tilley, DG; Chen, J; Le Corvoisier, P; Violin, JD; Wei, H; Lefkowitz, RJ; Rockman, HA. Beta-arrestin-mediated beta1-adrenergic receptor transactivation of the EGFR confers cardioprotection. Journal of Clinical Investigation. 2007;117:2445-2458. (2007) Abstract
Perrino, C; Naga Prasad, SV; Mao, L; Noma, T; Yan, Z; Kim, HS; Smithies, O; Rockman, HA. Intermittent pressure overload triggers hypertrophy-independent cardiac dysfunction and vascular rarefaction. Journal of Clinical Investigation. 2006;116:1547-1560. (2006) Abstract
Wolf, MJ; Amrein, H; Izatt, JA; Choma, MA; Reedy, MC; Rockman, HA. Drosophila as a model for the identification of genes causing adult human heart disease. Proceedings of the National Academy of Sciences of USA. 2006;103:1394-1399. (2006) Abstract
Naga Prasad, SV; Jayatilleke, A; Madamanchi, A; Rockman, HA. Protein kinase activity of phosphoinositide 3-kinase regulates beta-adrenergic receptor endocytosis. Nature Cell Biology. 2005;7:785-796. (2005) Abstract
Nienaber, JJ; Tachibana, H; Naga Prasad, SV; Esposito, G; Wu, D; Mao, L; Rockman, HA. Inhibition of receptor-localized PI3K preserves cardiac beta-adrenergic receptor function and ameliorates pressure overload heart failure. Journal of Clinical Investigation. 2003;112:1067-1079. (2003) Abstract