Physicians

Director, Flow Cytometry Laboratory

Department / Division
Pathology / Pathology Clinical Services

Address
DUMC 3712
Durham, NC 27710

Send consultative material to Carol Tate, M345 Davison Building, Durham, NC 27710

Appointment Telephone
919-684-2486

Office Telephone
919-668-0921

Fax Telephone
919-684-1856

Training

• MD, University of Pune B.J. Medical College (India), 1980

Residency

• Anatomic and Clinical Pathology, University of Mississippi Medical Center, 1994-1998

Fellowship

• Hematopathology, University of Wisconsin Hospital, 1998-1999

Other Training

• PhD, Immunology, University of Texas Health Science Center, 1989

Clinical Interests
Diagnostic consultation in leukemias, lymphomas, and other blood diseases; flow cytometric immunophenotyping; cell-subset analysis in HIV infection; cytogenetic and molecular diagnosis of congenital disorders and cancers; diagnostic consultations in diseases of the liver and biliary systems

Research Interests
My research interests are in the area of lymphomas and leukemias.

1. Multiparameter flow cytometry in the diagnosis and prognosis of hematopoietic and lymphoid neoplasm : We are studying the role of multiparameter flow cytometry in diagnosis of a) acute myeloid leukemias with myelodysplasia associated changes; b)acute myeloid leukemias with monocytic differentiation; c) chronic lymphocytic leukemia with atypical immunophenotype; d) ZAP70 and other prognostic markers in chronic lymphocytic leukemia; e) T/ NK cell lymphomas in spleen.

2. Extranodal lymphomas: The two areas of special interest are ocular adnexa and orbital tissue and liver. Retrospective analysis of a large series of ocular adnexal lymphomas was completed. Similarly hepatic lymphomas will be classified according to the current WHO criteria.

3. Biology of non-myeloablative allogeneic stem cell transplantation: Role of morphological and molecular diagnostic methods in appropriate care of patients undergoing this mode of treatment.

Industry Relationships and Collaborations (What's this?)

This faculty member has no reported relationships with industry.

Representative Publications
Cardona, DM; Layne, A; Lagoo, AS. Lymphomas of the gastro-intestinal tract - pathophysiology, pathology, and differential diagnosis. Indian Journal of Pathology and Microbiology. 2012;55:1-16. (2012) Abstract

Advani, AS; Gibson, S; Douglas, E; Diacovo, J; Elson, P; Kalaycio, M; Copelan, E; Sekeres, M; Sobecks, R; Sungren, S; Lagoo, A; Rizzieri, D; Hsi, E. Histone H4 acetylation by immunohistochemistry and prognosis in relapsed acute lymphocytic leukaemia (ALL). British Journal of Haematology. 2011;153:504-507. (2011) Abstract

Wang, E; Hutchinson, CB; Huang, Q; Lu, CM; Crow, J; Wang, FF; Sebastian, S; Rehder, C; Lagoo, A; Horwitz, M; Rizzieri, D; Yu, J; Goodman, B; Datto, M; Buckley, P. Donor cell-derived leukemias/myelodysplastic neoplasms in allogeneic hematopoietic stem cell transplant recipients: a clinicopathologic study of 10 cases and a comprehensive review of the literature. American Journal of Clinical Pathology. 2011;135:525-540. (2011) Abstract

Hassanein, NM; Perkinson, KR; Alcancia, F; Goodman, BK; Weinberg, JB; Lagoo, AS. A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia. American Journal of Clinical Pathology. 2010;133:708-717. (2010) Abstract

Ito, T; Kwon, HY; Zimdahl, B; Congdon, KL; Blum, J; Lento, WE; Zhao, C; Lagoo, A; Gerrard, G; Foroni, L; Goldman, J; Goh, H; Kim, SH; Kim, DW; Chuah, C; Oehler, VG; Radich, JP; Jordan, CT; Reya, T. Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature. 2010;466:765-768. (2010) Abstract

Jima, DD; Zhang, J; Jacobs, C; Richards, KL; Dunphy, CH; Choi, WW; Au, WY; Srivastava, G; Czader, MB; Rizzieri, DA; Lagoo, AS; Lugar, PL; Mann, KP; Flowers, CR; Bernal-Mizrachi, L; Naresh, KN; Evens, AM; Gordon, LI; Luftig, M; Friedman, DR; Weinberg, JB; Thompson, MA; Gill, JI; Liu, Q; How, T; Grubor, V; Gao, Y; Patel, A; Wu, H; Zhu, J; Blobe, GC; Lipsky, PE; Chadburn, A; Dave, SS; Hematologic Malignancies Research Consortium. Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs. Blood. 2010;116:e118-e127. (2010) Abstract

Pan, H; Ding, E; Hu, M; Lagoo, AS; Datto, MB; Lagoo-Deenadayalan, SA. SMAD4 is required for development of maximal endotoxin tolerance. Journal of Immunology. 2010;184:5502-5509. (2010) Abstract

Hassanein, NM; Alcancia, F; Perkinson, KR; Buckley, PJ; Lagoo, AS. Distinct expression patterns of CD123 and CD34 on normal bone marrow B-cell precursors ("hematogones") and B lymphoblastic leukemia blasts. American Journal of Clinical Pathology. 2009;132:573-580. (2009) Abstract

Li, Y; Howell, EA; Lagoo, AS; Kuchibhatla, M; Pan, H; Cohen, HJ; Lagoo, SA. Differential gene expression of interleukin-1 receptor associated kinase-1 and interleukin-1 receptor associated kinase-M in peripheral blood mononuclear cells of young and aged rats following preconditioning with endotoxin. Shock. 2009;31:55-63. (2009) Abstract

Rizzieri, DA; O'Brien, JA; Broadwater, G; Decastro, CM; Dev, P; Diehl, L; Beaven, A; Lagoo, A; Gockerman, JP; Chao, NJ; Moore, JO. Outcomes of patients who undergo aggressive induction therapy for secondary acute myeloid leukemia. Cancer. 2009;115:2922-2929. (2009) Abstract

Zhang, J; Jima, DD; Jacobs, C; Fischer, R; Gottwein, E; Huang, G; Lugar, PL; Lagoo, AS; Rizzieri, DA; Friedman, DR; Weinberg, JB; Lipsky, PE; Dave, SS. Patterns of microRNA expression characterize stages of human B-cell differentiation. Blood. 2009;113:4586-4594. (2009) Abstract

Lagoo, AS; Haggerty, C; Kim, Y; Hammons, M; Neufeld, K; Redher, C; Woodward, J; Klintworth, GK. Morphologic features of 115 lymphomas of the orbit and ocular adnexa categorized according to the World Health Organization classification: are marginal zone lymphomas in the orbit mucosa-associated lymphoid tissue-type lymphomas?. Archives of Pathology and Laboratory Medicine. 2008;132:1405-1416. (2008) Abstract

Mori, S; Rempel, RE; Chang, JT; Yao, G; Lagoo, AS; Potti, A; Bild, A; Nevins, JR. Utilization of pathway signatures to reveal distinct types of B lymphoma in the Emicro-myc model and human diffuse large B-cell lymphoma. Cancer Research. 2008;68:8525-8534. (2008) Abstract

Yang, DT; Dunphy, CH; Tripp, SR; Lagoo, AS; Perkins, SL. Nodular lymphocyte predominant Hodgkin lymphoma at atypical locations may be associated with increased numbers of large cells and a diffuse histologic component. American Journal of Hematology. 2008;83:218-221. (2008) Abstract

Karikari, IO; Thomas, KK; Lagoo, A; Cummings, TJ; George, TM. Primary cerebral ALK-1-positive anaplastic large cell lymphoma in a child. Case report and literature review. Pediatric Neurosurgery. 2007;43:516-521. (2007) Abstract

Sevilla, DW; Gong, JZ; Goodman, BK; Buckley, PJ; Rosoff, P; Gockerman, JP; Lagoo, AS. Clinicopathologic findings in high-grade B-cell lymphomas with typical Burkitt morphologic features but lacking the MYC translocation. American Journal of Clinical Pathology. 2007;128:981-991. (2007) Abstract

Lagoo, AS; Gong, JZ; Stenzel, TT; Goodman, BK; Buckley, PJ; Chao, NJ; Gasparetto, C; Long, GD; Rizzieri, DA. Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment. Archives of Pathology and Laboratory Medicine. 2006;130:1479-1488. (2006) Abstract

Lagoo, AS; Robboy, SJ. Lymphoma of the female genital tract: current status. International Journal of Gynecological Pathology. 2006;25:1-21. (2006) Abstract

Castellano-Sanchez, AA; Li, S; Qian, J; Lagoo, A; Weir, E; Brat, DJ. Primary central nervous system posttransplant lymphoproliferative disorders. American Journal of Clinical Pathology. 2004;121:246-253. (2004) Abstract

Rizzieri, DA; Akabani, G; Zalutsky, MR; Coleman, RE; Metzler, SD; Bowsher, JE; Toaso, B; Anderson, E; Lagoo, A; Clayton, S; Pegram, CN; Moore, JO; Gockerman, JP; DeCastro, C; Gasparetto, C; Chao, NJ; Bigner, DD. Phase 1 trial study of 131I-labeled chimeric 81C6 monoclonal antibody for the treatment of patients with non-Hodgkin lymphoma. Blood. 2004;104:642-648. (2004) Abstract

Safley, AM; Buckley, PJ; Creager, AJ; Dash, RC; Dodd, LG; Goodman, BK; Jones, CK; Lagoo, AS; Stenzel, TT; Wang, W; Xie, B; Gong, JZ. The value of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis of B-cell non-Hodgkin lymphoma by fine-needle aspiration. Archives of Pathology and Laboratory Medicine. 2004;128:1395-1403. (2004) Abstract

Gong, JZ; Lagoo, AS; Peters, D; Horvatinovich, J; Benz, P; Buckley, PJ. Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. American Journal of Clinical Pathology. 2001;116:893-897. (2001) Abstract

Lagoo, AS; Buckley, PJ; Burchell, LJ; Peters, D; Fechner, JH; Tsuchida, M; Dong, Y; Hong, X; Brunner, KG; Oberley, TD; Hamawy, MM; Knechtle, SJ. Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts. Transplantation. 2000;70:877-886. (2000) Abstract

McMurray, RW; Wilson, JG; Bigler, L; Xiang, L; Lagoo, A. Progesterone inhibits glucocorticoid-induced murine thymocyte apoptosis. International Journal of Immunopharmacology. 2000;22:955-965. (2000) Abstract

Lorenz, HM; Lagoo, AS; Lagoo-Deenadalayan, SA; Barber, WH; Kalden, JR; Hardy, KJ. Epitope-specific signaling through CD45 on T lymphocytes leads to cAMP synthesis in monocytes after ICAM-1-dependent cellular interaction. European Journal of Immunology. 1998;28:2300-2310. (1998) Abstract

Lagoo, AS; George, JF; Naftel, DC; Griffin, AK; Kirklin, JK; Lagoo-Deenadayalan, S; Hardy, KJ; Savunen, T; McGiffin, DC. Semiquantitative measurement of cytokine messenger RNA in endomyocardium and peripheral blood mononuclear cells from human heart transplant recipients. Journal of Heart and Lung Transplantation. 1996;15:206-217. (1996) Abstract

Everson, MP; Lagoo, A; McDuffie, DS; Pillion, DJ. Misoprostol Modulation of Dendritic Cell Function in Immune Responses. American Journal of Therapeutics. 1995;2:626-629. (1995) Abstract

Lagoo-Deenadayalan, S; Lagoo, AS; Lemons, JA; Lorenz, HM; Bass, JD; McDaniel, DO; Hardy, KJ; Barber, WH. Donor specific bone marrow cells suppress lymphocyte reactivity to donor antigens and differentially modulate TH1 and TH2 cytokine gene expression in the responder cell population. Transplant Immunology. 1995;3:124-134. (1995) Abstract

Lagoo, AS; Eldridge, JH; Lagoo-Deenadaylan, S; Black, CA; Ridwan, BU; Hardy, KJ; McGhee, JR; Beagley, KW. Peyer's patch CD8+ memory T cells secrete T helper type 1 and type 2 cytokines and provide help for immunoglobulin secretion. European Journal of Immunology. 1994;24:3087-3092. (1994) Abstract

Lagoo, AS; Lagoo-Deenadayalan, S; Lorenz, HM; Byrne, J; Barber, WH; Hardy, KJ. IL-2, IL-4, and IFN-gamma gene expression versus secretion in superantigen-activated T cells. Distinct requirement for costimulatory signals through adhesion molecules. Journal of Immunology. 1994;152:1641-1652. (1994) Abstract

Lorenz, HM; Lagoo, AS; Hardy, KJ. The cell and molecular basis of leukocyte common antigen (CD45)-triggered, lymphocyte function-associated antigen-1-/intercellular adhesion molecule-1-dependent, leukocyte adhesion. Blood. 1994;83:1862-1870. (1994) Abstract

Lagoo-Deenadayalan, S; Lagoo, AS; Barber, WH; Hardy, KJ. A standardized approach to PCR-based semiquantitation of multiple cytokine gene transcripts from small cell samples. Lymphokine and Cytokine Research. 1993;12:59-67. (1993) Abstract

Lagoo, A; Tseng, CK; Sell, S. Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine. Cytokine. 1990;2:272-279. (1990) Abstract

Lagoo, A; Tseng, CK; Sell, S. Molecular signals in B cell activation. I. Differential refractory effects of incomplete signaling by ionomycin or PMA relate to autocrine IL-2 production and IL-2R expression. Cellular Immunology. 1990;127:483-496. (1990) Abstract

Lagoo, A; Tseng, CK; Sell, S. Molecular signals in B cell activation. II. IL-2-mediated signals are required in late G1 for transition to S phase after ionomycin and PMA treatment. Cellular Immunology. 1990;127:497-505. (1990) Abstract

Lagoo, A; Sell, S. Early activation and cell cycle entry of resting B cells after Fab-anti-Ig treatment: role of receptor crosslinking. Cellular Immunology. 1989;118:53-67. (1989) Abstract