Anand S. Lagoo, MD, PhD

Director, Flow Cytometry Laboratory

Department / Division:
Pathology / Pathology

Address:
DUMC 3712
Durham, NC 27710

Send consultative material to Carol Tate, M255 Davison Building, Durham, NC 27710

Appointment Telephone:
919-684-2486

Office Telephone:
919-668-0921

Fax Telephone:
919-684-1856

• MD, B.J. Medical College and University of Poona (India), 1980

• Anatomic and Clinical Pathology, University of Mississippi Medical Center, 1994-1998

• Hematopathology, University of Wisconsin Hospital, 1998-1999

• PhD, Immunology, University of Texas Health Science Center, 1989

Clinical Interests:
Diagnostic consultation in blood cancers (leukemias), lymphomas and other blood diseases, flow cytometric immunophenotyping, cell subset analysis in HIV infection, cytogenetic and molecular diagnosis of congenital disorders and cancers, diagnostic consultations in diseases of the liver and biliary systems

Research Interests:
My research interests are in the area of lymphomas and leukemias.

1. Biology of variant sub-populations in acute leukemia:  Multiparameter flow cytometry coupled with cell sorting provide a very powerful tool to analyze small subpopulations in acute leukemia cells.  Such subpopulations are defined based on aberrant expression of certain surface antigens.  The biological behavior of these subpopulations is largely unknown.  Acute lymphoblastic leukemia, the commonest cancer of childhood, often shows variable levels of expression of non-lymphoid antigens on some of the leukemia cells.  Conversely, acute myeloid leukemias show variable expression of lymphoid antigens on a proportion of leukemic cells. Whether these differences represent true subclones is not clear. Multiparameter flow cytometry will be used to identify a subset of these cases where the aberrant expression occurs on a distinct sub-population.  These cells will be isolated by cell sorting and compared to the dominant population as regards growth rate, cytogenetic abnormalities, growth factor requirement if any, in-vitro response to cytotoxic drugs and expression of selected oncogenes.  These cases will be clinically followed up to examine the fate of these subpopulations following therapy and when it occurs, in relapse.

2. Lymphoid lesions of the ocular adnexa: We are analyzing a large collection of lymphomas occurring in and around the eye. The marginal zone lymphomas of the MALT type will be studied extensively to determine the morphological, immunophenotypical, and cytogenetic properties that can predict behavior. In particular, the cytogenetic abnormalities associated with poor outcome in gastric MALT lymphomas will be studied in archival tissues using fluorescent in-situ hybridization and PCR.

3. Biology of non-myeloablative allogeneic stem cell transplantation: Increasing numbers of hematological, lymphoid, and solid tumors are being treated with this approach using reduced intensity conditioning regimens. I am interested in correlating morphological examination of the bone marrow following transplantation, the level of donor engraftment as judged by molecular methods, and patient outcome.

Representative Publications:
Zhang J, Jima DD, Jacobs C, Fischer R, Gottwein E, Huang G, Lugar PL, Lagoo AS, Rizzieri DA, Friedman DR, Weinberg JB, Lipsky PE, Dave SS. Patterns of microRNA expression characterize stages of human B-cell differentiation. Blood. 2009 May 7;113(19):4586-94. (2009) Abstract

Li Y, Howell EA, Lagoo AS, Kuchibhatla M, Pan H, Cohen HJ, Lagoo SA. Differential gene expression of interleukin-1 receptor associated kinase-1 and interleukin-1 receptor associated kinase-M in peripheral blood mononuclear cells of young and aged rats following preconditioning with endotoxin. Shock. 2009 Jan;31(1):55-63. (2009) Abstract

Mori S, Rempel RE, Chang JT, Yao G, Lagoo AS, Potti A, Bild A, Nevins JR. Utilization of pathway signatures to reveal distinct types of B lymphoma in the Emicro-myc model and human diffuse large B-cell lymphoma. Cancer Res. 2008 Oct 15;68(20):8525-34. (2008) Abstract

Lagoo AS, Haggerty C, Kim Y, Hammons M, Neufeld K, Redher C, Woodward J, Klintworth GK. Morphologic features of 115 lymphomas of the orbit and ocular adnexa categorized according to the World Health Organization classification: are marginal zone lymphomas in the orbit mucosa-associated lymphoid tissue-type lymphomas? Arch Pathol Lab Med. 2008 Sep;132(9):1405-16. (2008) Abstract

Yang DT, Dunphy CH, Tripp SR, Lagoo AS, Perkins SL. Nodular lymphocyte predominant Hodgkin lymphoma at atypical locations may be associated with increased numbers of large cells and a diffuse histologic component. Am J Hematol. 2007 Oct 4. (2007) Abstract

Sevilla DW, Gong JZ, Goodman BK, Buckley PJ, Rosoff P, Gockerman JP, Lagoo AS. Clinicopathologic findings in high-grade B-cell lymphomas with typical Burkitt morphologic features but lacking the MYC translocation. Am J Clin Pathol. 2007 Dec;128(6):981-91. (2007) Abstract

Rizzieri DA, Koh LP, Long GD, Gasparetto C, Sullivan KM, Horwitz M, Chute J, Smith C, Gong JZ, Lagoo A, Niedzwiecki D, Dowell JM, Waters-Pick B, Liu C, Marshall D, Vredenburgh JJ, Gockerman J, Decastro C, Moore J, Chao NJ. Partially matched, nonmyeloablative allogeneic transplantation: clinical outcomes and immune reconstitution. J Clin Oncol. 2007 Feb 20;25(6):690-7. (2007) Abstract

Karikari IO, Thomas KK, Lagoo A, Cummings TJ, George TM. Primary cerebral ALK-1-positive anaplastic large cell lymphoma in a child. Case report and literature review. Pediatr Neurosurg. 2007;43(6):516-21. (2007) Abstract

Lagoo AS, Robboy SJ. Lymphoma of the female genital tract: current status. Int J Gynecol Pathol. 2006 Jan;25(1):1-21. (2006) Abstract

Lagoo AS, Gong JZ, Stenzel TT, Goodman BK, Buckley PJ, Chao NJ, Gasparetto C, Long GD, Rizzieri DA. Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment. Arch Pathol Lab Med. 2006 Oct;130(10):1479-88. (2006) Abstract

Rizzieri DA, Akabani G, Zalutsky MR, Coleman RE, Metzler SD, Bowsher JE, Toaso B, Anderson E, Lagoo A, Clayton S, Pegram CN, Moore JO, Gockerman JP, DeCastro C, Gasparetto C, Chao NJ, Bigner DD. Phase 1 trial study of 131I-labeled chimeric 81C6 monoclonal antibody for the treatment of patients with non-Hodgkin lymphoma. Blood. 2004 Aug 1;104(3):642-8. (2004) Abstract

Gong JZ, Snyder MJ, Lagoo AS, Vollmer RT, Dash RR, Madden JF, Buckley PJ, Jones CK. Diagnostic impact of core-needle biopsy on fine-needle aspiration of non-Hodgkin lymphoma. Diagn Cytopathol. 2004 Jul;31(1):23-30. (2004) Abstract

Castellano-Sanchez AA, Li S, Qian J, Lagoo A, Weir E, Brat DJ. Primary central nervous system posttransplant lymphoproliferative disorders. Am J Clin Pathol. 2004 Feb;121(2):246-53. (2004) Abstract

Safford SD, Lagoo AS, Mahaffey SA. Preoperative embolization as an adjunct to the operative management of mediastinal castleman disease. J Pediatr Surg. 2003 Sep;38(9):E21-3. (2003) Abstract

Gong JZ, Stenzel TT, Bennett ER, Lagoo AS, Dunphy CH, Moore JO, Rizzieri DA, Tepperberg JH, Papenhausen P, Buckley PJ. Burkitt lymphoma arising in organ transplant recipients: a clinicopathologic study of five cases. Am J Surg Pathol. 2003 Jun;27(6):818-27. (2003) Abstract

Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ. Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am J Clin Pathol. 2001 Dec;116(6):893-7. (2001) Abstract

McMurray RW, Wilson JG, Bigler L, Xiang L, Lagoo A. Progesterone inhibits glucocorticoid-induced murine thymocyte apoptosis. Int J Immunopharmacol. 2000 Nov;22(11):955-65. (2000) Abstract

Lagoo AS, Buckley PJ, Burchell LJ, Peters D, Fechner JH, Tsuchida M, Dong Y, Hong X, Brunner KG, Oberley TD, Hamawy MM, Knechtle SJ. Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts. Transplantation. 2000 Sep 27;70(6):877-86. (2000) Abstract

McMurray RW, Elbourne KB, Lagoo A, Lal S. Mycophenolate mofetil suppresses autoimmunity and mortality in the female NZB x NZW F1 mouse model of systemic lupus erythematosus. J Rheumatol. 1998 Dec;25(12):2364-70. (1998) Abstract

Lorenz HM, Lagoo AS, Lagoo-Deenadalayan SA, Barber WH, Kalden JR, Hardy KJ. Epitope-specific signaling through CD45 on T lymphocytes leads to cAMP synthesis in monocytes after ICAM-1-dependent cellular interaction. Eur J Immunol. 1998 Aug;28(8):2300-10. (1998) Abstract

Lagoo-Deenadayalan S, Lagoo AS, Lemons JA, Lorenz HM, Bass JD, McDaniel DO, Hardy KJ, Barber WH. Donor-specific bone marrow cells suppress lymphocyte reactivity to donor antigens and differentially modulate TH1 and TH2 cytokine gene expression in the responder cell population. Adv Nephrol Necker Hosp. 1996;25:107-27. (1996) Abstract

Lagoo AS, George JF, Naftel DC, Griffin AK, Kirklin JK, Lagoo-Deenadayalan S, Hardy KJ, Savunen T, McGiffin DC. Semiquantitative measurement of cytokine messenger RNA in endomyocardium and peripheral blood mononuclear cells from human heart transplant recipients. J Heart Lung Transplant. 1996 Feb;15(2):206-17. (1996) Abstract

Lagoo-Deenadayalan S, Lagoo AS, Lemons JA, Lorenz HM, Bass JD, McDaniel DO, Hardy KJ, Barber WH. Donor specific bone marrow cells suppress lymphocyte reactivity to donor antigens and differentially modulate TH1 and TH2 cytokine gene expression in the responder cell population. Transpl Immunol. 1995 Jun;3(2):124-34. (1995) Abstract

Kriegsmann J, Keyszer GM, Geiler T, Lagoo AS, Lagoo-Deenadayalan S, Gay RE, Gay S. Expression of E-selectin messenger RNA and protein in rheumatoid arthritis. Arthritis Rheum. 1995 Jun;38(6):750-4. (1995) Abstract

Hauser CJ, Lagoo S, Lagoo A, Hale E, Hardy KJ, Barber WH, Bass JD, Poole GV. Human peripheral mononuclear cells do not show proinflammatory patterns of cytokine transcription in early trauma: a preliminary report. Shock. 1995 Oct;4(4):247-50. (1995) Abstract

Everson MP, Lagoo A, McDuffie DS, Pillion DJ. Misoprostol Modulation of Dendritic Cell Function in Immune Responses. Am J Ther. 1995 Sep;2(9):626-629. (1995) Abstract

Beagley KW, Fujihashi K, Lagoo AS, Lagoo-Deenadaylan S, Black CA, Murray AM, Sharmanov AT, Yamamoto M, McGhee JR, Elson CO. Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine. J Immunol. 1995 Jun 1;154(11):5611-9. (1995) Abstract

Lorenz HM, Lagoo AS, Hardy KJ. The cell and molecular basis of leukocyte common antigen (CD45)-triggered, lymphocyte function-associated antigen-1-/intercellular adhesion molecule-1-dependent, leukocyte adhesion. Blood. 1994 Apr 1;83(7):1862-70. (1994) Abstract

Lagoo AS, Lagoo-Deenadayalan S, Lorenz HM, Byrne J, Barber WH, Hardy KJ. IL-2, IL-4, and IFN-gamma gene expression versus secretion in superantigen-activated T cells. Distinct requirement for costimulatory signals through adhesion molecules. J Immunol. 1994 Feb 15;152(4):1641-52. (1994) Abstract

Lagoo AS, Eldridge JH, Lagoo-Deenadaylan S, Black CA, Ridwan BU, Hardy KJ, McGhee JR, Beagley KW. Peyer's patch CD8+ memory T cells secrete T helper type 1 and type 2 cytokines and provide help for immunoglobulin secretion. Eur J Immunol. 1994 Dec;24(12):3087-92. (1994) Abstract

Lagoo-Deenadayalan S, Lagoo AS, Barber WH, Hardy KJ. A standardized approach to PCR-based semiquantitation of multiple cytokine gene transcripts from small cell samples. Lymphokine Cytokine Res. 1993 Apr;12(2):59-67. (1993) Abstract

Lagoo-Deenadayalan S, Lagoo AS, Hardy KJ, Grimm EA. Tumor recognition and lytic competence of IL-2-activated lymphocytes: regulation of both antibody-independent and -dependent cellular cytotoxicity via P75 IL-2 receptor. Lymphokine Cytokine Res. 1992 Aug;11(4):207-13. (1992) Abstract

Lagoo A, Tseng CK, Sell S. Molecular signals in B cell activation. II. IL-2-mediated signals are required in late G1 for transition to S phase after ionomycin and PMA treatment. Cell Immunol. 1990 May;127(2):497-505. (1990) Abstract

Lagoo A, Tseng CK, Sell S. Molecular signals in B cell activation. I. Differential refractory effects of incomplete signaling by ionomycin or PMA relate to autocrine IL-2 production and IL-2R expression. Cell Immunol. 1990 May;127(2):483-96. (1990) Abstract

Lagoo A, Tseng CK, Sell S. Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine. Cytokine. 1990 Jul;2(4):272-9. (1990) Abstract

Lagoo A, Sell S. Early activation and cell cycle entry of resting B cells after Fab-anti-Ig treatment: role of receptor crosslinking. Cell Immunol. 1989 Jan;118(1):53-67. (1989) Abstract