Physicians

Director, Flow Cytometry Laboratory

Department / Division
Pathology / Pathology Clinical Services

Address
DUMC 3712
Durham, NC 27710

Send consultative material to Carol Tate, M345 Davison Building, Durham, NC 27710

Appointment Telephone
919-684-2486

Office Telephone
919-668-0921

Fax Telephone
919-684-1856

Training

• MD, B.J. Medical College and University of Poona (India), 1980

Residency

• Anatomic and Clinical Pathology, University of Mississippi Medical Center, 1994-1998

Fellowship

• Hematopathology, University of Wisconsin Hospital, 1998-1999

Other Training

• PhD, Immunology, University of Texas Health Science Center, 1989

Clinical Interests
Diagnostic consultation in leukemias, lymphomas, and other blood diseases; flow cytometric immunophenotyping; cell-subset analysis in HIV infection; cytogenetic and molecular diagnosis of congenital disorders and cancers; diagnostic consultations in diseases of the liver and biliary systems

Research Interests
My research interests are in the area of lymphomas and leukemias.

1. Multiparameter flow cytometry in the diagnosis and prognosis of hematopoietic and lymphoid neoplasm : We are studying the role of multiparameter flow cytometry in diagnosis of a) acute myeloid leukemias with myelodysplasia associated changes; b)acute myeloid leukemias with monocytic differentiation; c) chronic lymphocytic leukemia with atypical immunophenotype; d) ZAP70 and other prognostic markers in chronic lymphocytic leukemia; e) T/ NK cell lymphomas in spleen.

2. Extranodal lymphomas: The two areas of special interest are ocular adnexa and orbital tissue and liver. Retrospective analysis of a large series of ocular adnexal lymphomas was completed. Similarly hepatic lymphomas will be classified according to the current WHO criteria.

3. Biology of non-myeloablative allogeneic stem cell transplantation: Role of morphological and molecular diagnostic methods in appropriate care of patients undergoing this mode of treatment.

Industry Relationships and Collaborations (What's this?)

This physician (or a member of their immediate family) has a working relationship (i.e. consulting, research, and/or educational services) with the companies listed below. These relations have been reported to the health system leadership and, when appropriate, management plans are in place to address potential conflicts.

• Per Group LP
• St Georges University, Grenada, West Indies

Representative Publications
Hassanein NM, Perkinson KR, Alcancia F, Goodman BK, Weinberg JB, Lagoo AS. A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia. Am J Clin Pathol. 2010 May;133(5):708-17. (2010) Abstract

Hassanein NM, Alcancia F, Perkinson KR, Buckley PJ, Lagoo AS. Distinct expression patterns of CD123 and CD34 on normal bone marrow B-cell precursors ("hematogones") and B lymphoblastic leukemia blasts. Am J Clin Pathol. 2009 Oct;132(4):573-80. (2009) Abstract

Li Y, Howell EA, Lagoo AS, Kuchibhatla M, Pan H, Cohen HJ, Lagoo SA. Differential gene expression of interleukin-1 receptor associated kinase-1 and interleukin-1 receptor associated kinase-M in peripheral blood mononuclear cells of young and aged rats following preconditioning with endotoxin. Shock. 2009 Jan;31(1):55-63. (2009) Abstract

Rizzieri DA, O'Brien JA, Broadwater G, Decastro CM, Dev P, Diehl L, Beaven A, Lagoo A, Gockerman JP, Chao NJ, Moore JO. Outcomes of patients who undergo aggressive induction therapy for secondary acute myeloid leukemia. Cancer. 2009 Jul 1;115(13):2922-9. (2009) Abstract

Zhang J, Jima DD, Jacobs C, Fischer R, Gottwein E, Huang G, Lugar PL, Lagoo AS, Rizzieri DA, Friedman DR, Weinberg JB, Lipsky PE, Dave SS. Patterns of microRNA expression characterize stages of human B-cell differentiation. Blood. 2009 May 7;113(19):4586-94. (2009) Abstract

Lagoo AS, Haggerty C, Kim Y, Hammons M, Neufeld K, Redher C, Woodward J, Klintworth GK. Morphologic features of 115 lymphomas of the orbit and ocular adnexa categorized according to the World Health Organization classification: are marginal zone lymphomas in the orbit mucosa-associated lymphoid tissue-type lymphomas? Arch Pathol Lab Med. 2008 Sep;132(9):1405-16. (2008) Abstract

Mori S, Rempel RE, Chang JT, Yao G, Lagoo AS, Potti A, Bild A, Nevins JR. Utilization of pathway signatures to reveal distinct types of B lymphoma in the Emicro-myc model and human diffuse large B-cell lymphoma. Cancer Res. 2008 Oct 15;68(20):8525-34. (2008) Abstract

Karikari IO, Thomas KK, Lagoo A, Cummings TJ, George TM. Primary cerebral ALK-1-positive anaplastic large cell lymphoma in a child. Case report and literature review. Pediatr Neurosurg. 2007;43(6):516-21. (2007) Abstract

Sevilla DW, Gong JZ, Goodman BK, Buckley PJ, Rosoff P, Gockerman JP, Lagoo AS. Clinicopathologic findings in high-grade B-cell lymphomas with typical Burkitt morphologic features but lacking the MYC translocation. Am J Clin Pathol. 2007 Dec;128(6):981-91. (2007) Abstract

Yang DT, Dunphy CH, Tripp SR, Lagoo AS, Perkins SL. Nodular lymphocyte predominant Hodgkin lymphoma at atypical locations may be associated with increased numbers of large cells and a diffuse histologic component. Am J Hematol. 2007 Oct 4. (2007) Abstract

Lagoo AS, Gong JZ, Stenzel TT, Goodman BK, Buckley PJ, Chao NJ, Gasparetto C, Long GD, Rizzieri DA. Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment. Arch Pathol Lab Med. 2006 Oct;130(10):1479-88. (2006) Abstract

Lagoo AS, Robboy SJ. Lymphoma of the female genital tract: current status. Int J Gynecol Pathol. 2006 Jan;25(1):1-21. (2006) Abstract

Castellano-Sanchez AA, Li S, Qian J, Lagoo A, Weir E, Brat DJ. Primary central nervous system posttransplant lymphoproliferative disorders. Am J Clin Pathol. 2004 Feb;121(2):246-53. (2004) Abstract

Rizzieri DA, Akabani G, Zalutsky MR, Coleman RE, Metzler SD, Bowsher JE, Toaso B, Anderson E, Lagoo A, Clayton S, Pegram CN, Moore JO, Gockerman JP, DeCastro C, Gasparetto C, Chao NJ, Bigner DD. Phase 1 trial study of 131I-labeled chimeric 81C6 monoclonal antibody for the treatment of patients with non-Hodgkin lymphoma. Blood. 2004 Aug 1;104(3):642-8. (2004) Abstract

Safley AM, Buckley PJ, Creager AJ, Dash RC, Dodd LG, Goodman BK, Jones CK, Lagoo AS, Stenzel TT, Wang W, Xie B, Gong JZ. The value of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis of B-cell non-Hodgkin lymphoma by fine-needle aspiration. Arch Pathol Lab Med. 2004 Dec;128(12):1395-403. (2004) Abstract

Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ. Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am J Clin Pathol. 2001 Dec;116(6):893-7. (2001) Abstract

Lagoo AS, Buckley PJ, Burchell LJ, Peters D, Fechner JH, Tsuchida M, Dong Y, Hong X, Brunner KG, Oberley TD, Hamawy MM, Knechtle SJ. Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts. Transplantation. 2000 Sep 27;70(6):877-86. (2000) Abstract

McMurray RW, Wilson JG, Bigler L, Xiang L, Lagoo A. Progesterone inhibits glucocorticoid-induced murine thymocyte apoptosis. Int J Immunopharmacol. 2000 Nov;22(11):955-65. (2000) Abstract

Lorenz HM, Lagoo AS, Lagoo-Deenadalayan SA, Barber WH, Kalden JR, Hardy KJ. Epitope-specific signaling through CD45 on T lymphocytes leads to cAMP synthesis in monocytes after ICAM-1-dependent cellular interaction. Eur J Immunol. 1998 Aug;28(8):2300-10. (1998) Abstract

Lagoo AS, George JF, Naftel DC, Griffin AK, Kirklin JK, Lagoo-Deenadayalan S, Hardy KJ, Savunen T, McGiffin DC. Semiquantitative measurement of cytokine messenger RNA in endomyocardium and peripheral blood mononuclear cells from human heart transplant recipients. J Heart Lung Transplant. 1996 Feb;15(2):206-17. (1996) Abstract

Everson MP, Lagoo A, McDuffie DS, Pillion DJ. Misoprostol Modulation of Dendritic Cell Function in Immune Responses. Am J Ther. 1995 Sep;2(9):626-629. (1995) Abstract

Lagoo-Deenadayalan S, Lagoo AS, Lemons JA, Lorenz HM, Bass JD, McDaniel DO, Hardy KJ, Barber WH. Donor specific bone marrow cells suppress lymphocyte reactivity to donor antigens and differentially modulate TH1 and TH2 cytokine gene expression in the responder cell population. Transpl Immunol. 1995 Jun;3(2):124-34. (1995) Abstract

Lagoo AS, Eldridge JH, Lagoo-Deenadaylan S, Black CA, Ridwan BU, Hardy KJ, McGhee JR, Beagley KW. Peyer's patch CD8+ memory T cells secrete T helper type 1 and type 2 cytokines and provide help for immunoglobulin secretion. Eur J Immunol. 1994 Dec;24(12):3087-92. (1994) Abstract

Lagoo AS, Lagoo-Deenadayalan S, Lorenz HM, Byrne J, Barber WH, Hardy KJ. IL-2, IL-4, and IFN-gamma gene expression versus secretion in superantigen-activated T cells. Distinct requirement for costimulatory signals through adhesion molecules. J Immunol. 1994 Feb 15;152(4):1641-52. (1994) Abstract

Lorenz HM, Lagoo AS, Hardy KJ. The cell and molecular basis of leukocyte common antigen (CD45)-triggered, lymphocyte function-associated antigen-1-/intercellular adhesion molecule-1-dependent, leukocyte adhesion. Blood. 1994 Apr 1;83(7):1862-70. (1994) Abstract

Lagoo-Deenadayalan S, Lagoo AS, Barber WH, Hardy KJ. A standardized approach to PCR-based semiquantitation of multiple cytokine gene transcripts from small cell samples. Lymphokine Cytokine Res. 1993 Apr;12(2):59-67. (1993) Abstract

Lagoo A, Tseng CK, Sell S. Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine. Cytokine. 1990 Jul;2(4):272-9. (1990) Abstract

Lagoo A, Tseng CK, Sell S. Molecular signals in B cell activation. I. Differential refractory effects of incomplete signaling by ionomycin or PMA relate to autocrine IL-2 production and IL-2R expression. Cell Immunol. 1990 May;127(2):483-96. (1990) Abstract

Lagoo A, Tseng CK, Sell S. Molecular signals in B cell activation. II. IL-2-mediated signals are required in late G1 for transition to S phase after ionomycin and PMA treatment. Cell Immunol. 1990 May;127(2):497-505. (1990) Abstract

Lagoo A, Sell S. Early activation and cell cycle entry of resting B cells after Fab-anti-Ig treatment: role of receptor crosslinking. Cell Immunol. 1989 Jan;118(1):53-67. (1989) Abstract