Judith A. Voynow, MD

Department / Division:
Pediatrics / Pulmonary and Sleep Medicine

Address:
DUMC 2994
Durham, NC 27710

Appointment Telephone:
(919) 681-3364

Office Telephone:
(919) 684-3364

Fax Telephone:
(919) 684-2292

• M.D., University of Pennsylvania, 1982

• Pediatrics, Children's Hospital Philadelphia, Pennsylvania, 1982-1985

• Pediatric Pulmonary, Children's Hospital Philadelphia, Pennsylvania, 1985-1990

Clinical Interests:
Cystic fibrosis, pediatric sleep medicine, pediatric lung disease, flexible fiberoptic laryngoscopy and bronchoscopy, asthma, bronchopulmonary dysplasia, recurrent pneumonia

Research Interests:
    Cystic Fibrosis (CF) is the most common fatal inherited disease in Caucasians.  CF lung disease is characterized by mucus obstruction, and neutrophil-predominant inflammation of the airways.  The major macromolecular components of mucus are the mucin glycoproteins.  These large heavily glycosylated glycoproteins impart viscoelastic properties to mucus, and under normal conditions, are an important component of lung innate immunity.  However, in disease states like CF, increased mucin production and hypersecretion significantly impair pulmonary function and lead to chronic inflammation, infection and eventually lung damage.   Our laboratory has been studying the regulation of the major respiratory tract mucins and their functions in the lung.
    Recently, our work has focused on the regulation of mucins by neutrophil elastase (NE), a major inflammatory mediator in CF. We have found that NE increases expression of two major respiratory tract mucins, MUC5AC and MUC4, and this effect is mediated by oxidant stress.  Furthermore, we found that NE regulates these mucins by a post-transcriptional regulatory mechanism. Our current research endeavors include: 1) identification of the RNA-binding regulatory proteins for MUC expression, 2) analysis of the oxidants/pro-oxidant enzymes regulating MUC expression and 3)evaluation of the mechanisms of airway remodeling following NE exposure.

Representative Publications:
Adler KB, Fischer BM, Martin LD, Voynow JA (1998) Effects of inflammatory mediators and drugs on mucus secretion and mucociliary function. Res. Immunol. 149:245-248. (1998)

Giuntoli RL 2nd, Rodriguez GC, Whitaker RS, Dodge R, Voynow JA. Mucin gene expression in ovarian cancers. Cancer Res. 1998 Dec 1;58(23):5546-50. (1998) Abstract

Voynow JA, Young LR, Wang Y, Horger T, Rose MC, Fischer BM. Neutrophil elastase increases MUC5AC mRNA and protein expression in respiratory epithelial cells. Am J Physiol. 1999 May;276(5 Pt 1):L835-43. (1999) Abstract

Voynow JA, Charney EB. Fibrodysplasia ossificans progressiva presenting as osteomyelitis-like syndrome. Clin Pediatr (Phila). 1986 Jul;25(7):373-5. (1986) Abstract

Voynow JA, Selby DM, Rose MC. Mucin gene expression (MUC1, MUC2, and MUC5/5AC) in nasal epithelial cells of cystic fibrosis, allergic rhinitis, and normal individuals. Lung. 1998;176(5):345-54. (1998) Abstract

Voynow JA, Schanberg L, Sporn T, Kredich D. Pulmonary complications associated with Kawasaki disease. J Pediatr. 2002 Jun;140(6):786-7. (2002) Abstract

Martin LD, Krunkosky TM, Voynow JA, Adler KB (1998) Oxidant-regulated gene expression in inflammatory lung disease, in Acute Respiratory Distress Syndrome: Cellular and Molecular Mechanisms and Clinical Management, Matalon, S. and J.I. Sznajder, Eds., Plenum Press., NY, pp. 187-195. (1998)

Voynow JA, Rose MC. Quantitation of mucin mRNA in respiratory and intestinal epithelial cells. Am J Respir Cell Mol Biol. 1994 Dec;11(6):742-50. (1994) Abstract

Rose MC, Nickola TJ, Voynow JA. Airway mucus obstruction: mucin glycoproteins, MUC gene regulation and goblet cell hyperplasia. Am J Respir Cell Mol Biol. 2001 Nov;25(5):533-7. (2001) Abstract

Martin LD, Krunkosky TM, Voynow JA, Adler KB. The role of reactive oxygen and nitrogen species in airway epithelial gene expression. Environ Health Perspect. 1998 Oct;106 Suppl 5:1197-203. (1998) Abstract

Voynow JA, Kaiser RS, Scanlin TF, Glick MC. Purification and characterization of GDP-L-fucose-N-acetyl beta-D-glucosaminide alpha 1----6fucosyltransferase from cultured human skin fibroblasts. Requirement of a specific biantennary oligosaccharide as substrate. J Biol Chem. 1991 Nov 15;266(32):21572-7. (1991) Abstract

Scanlin TF, Voynow JA, Thomas EJ, Glick MC. Glycoproteins in culture medium: a comparison from cystic fibrosis and control skin fibroblasts. Biochemistry. 1982 Feb 2;21(3):491-7. (1982) Abstract

Voynow JA. What does mucin have to do with lung disease? Paediatr Respir Rev. 2002 Jun;3(2):98-103. (2002) Abstract

Berger JT, Voynow JA, Peters KW, Rose MC. Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Am J Respir Cell Mol Biol. 1999 Mar;20(3):500-10. (1999) Abstract

Voynow JA, Scanlin TF, Glick MC. A quantitative method for GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha 1----6fucosyltransferase activity with lectin affinity chromatography. Anal Biochem. 1988 Feb 1;168(2):367-73. (1988) Abstract

Fischer BM, Rochelle LG, Voynow JA, Akley NJ, Adler KB. Tumor necrosis factor-alpha stimulates mucin secretion and cyclic GMP production by guinea pig tracheal epithelial cells in vitro. Am J Respir Cell Mol Biol. 1999 Mar;20(3):413-22. (1999) Abstract

Fischer BM, Cuellar JG, Diehl ML, deFreytas AM, Zhang J, Carraway KL, Voynow JA. Neutrophil elastase increases MUC4 expression in normal human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2003 Apr;284(4):L671-9. (2003) Abstract

Fischer BM, Voynow JA. Neutrophil elastase induces MUC5AC gene expression in airway epithelium via a pathway involving reactive oxygen species. Am J Respir Cell Mol Biol. 2002 Apr;26(4):447-52. (2002) Abstract

Krunkosky TM, Martin LD, Fischer BM, Voynow JA, Adler KB. Effects of TNFalpha on expression of ICAM-1 in human airway epithelial cells in vitro: oxidant-mediated pathways and transcription factors. Free Radic Biol Med. 2003 Nov 1;35(9):1158-67. (2003) Abstract

Voynow JA, Gendler SJ, Rose MC. Regulation of mucin genes in chronic inflammatory airway diseases. Am J Respir Cell Mol Biol. 2006 Jun;34(6):661-5. (2006) Abstract

Voynow JA, Fischer BM, Roberts BC, Proia AD. Basal-like cells constitute the proliferating cell population in cystic fibrosis airways. Am J Respir Crit Care Med. 2005 Oct 15;172(8):1013-8. (2005) Abstract

Fischer BM, Cuellar JG, Byrd AS, Rice AB, Bonner JC, Martin LD, Voynow JA. ErbB2 activity is required for airway epithelial repair following neutrophil elastase exposure. FASEB J. 2005 Aug;19(10):1374-6. (2005) Abstract

Rose MC, Voynow JA. Respiratory tract mucin genes and mucin glycoproteins in health and disease. Physiol Rev. 2006 Jan;86(1):245-78. (2006) Abstract

Voynow JA, Fischer BM, Malarkey DE, Burch LH, Wong T, Longphre M, Ho SB, Foster WM. Neutrophil elastase induces mucus cell metaplasia in mouse lung. Am J Physiol Lung Cell Mol Physiol. 2004 Dec;287(6):L1293-302. (2004) Abstract

Chitano P, Voynow JA, Pozzato V, Cantillana V, Burch LH, Wang L, Murphy TM. Ontogenesis of myosin light chain kinase mRNA and protein content in guinea pig tracheal smooth muscle. Pediatr Pulmonol. 2004 Dec;38(6):456-64. (2004) Abstract

Zheng S, Kenan DJ, Fischer BM, Voynow JA. Neutrophil Elastase Increases MUC5AC Mucin mRNA Expression via Post-Transcriptional Regulation. Proc Am Thorac Soc. 2006 Aug;3(6):543b-544. (2006) Abstract